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Emergence of Pathogenic Strains of Staphylococcus aureus in Goat Milk and Their Comparative Response to Antibiotics

Emergence of Pathogenic Strains of Staphylococcus aureus in Goat Milk and Their Comparative Response to Antibiotics

Iqra Muzammil1, Muhammad Ijaz Saleem1, Amjad Islam Aqib2,*, Ambreen Ashar3Syed Ashar Mahfooz1, Sajjad ur Rahman4, Muhammad Shoaib4, Muhammad Aamir Naseer1, Imran Khan Sohrani1, Javeed Ahmad1, Razaullah Saqi1, Fizzah Laeeq Lodhi1 and
Qaisar Tanveer5

1Department of Clinical Medicine and Surgery, Faculty of Veterinary Science, University of Agriculture, Faisalabad-38000
2Department of Medicine, Faculty of Veterinary Science, Cholistan University of Veterinary and Animal Sciences, Bahawalpur-63100
3Government College for Women University, Faisalabad-38000
4Institute of Microbiology, Faculty of Veterinary Science, University of Agriculture, Faisalabad-38000
5Institute of Pharmacy, Physiology and Pharmacology, University of Agriculture, Faisalabad-38000

*      Corresponding author: amjadwaseer@gmail.com

ABSTRACT

The nutraceutical milk of goat in agrobased countries is at risk of contamination with pathogenic strains of Staphylococcus aureus. The current study was designed to investigate prevalence of pathogenic strains of S. aureus, assessment of risk factors, and in-vitro antibiogram of non-biofilm producing S. aureus (nbpSA) and biofilm positive S. aureus (bpSA)from mastitic goats. The purposive sampling technique was applied to collect n=200 milk samples from different regions of goat populated areas of district Faisalabad-Pakistan. Using surf field mastitis test, collected milk samples were screened for subclinical mastitis at the spot for subsequent identification of pathogenic strains of S. aureus through microbiological examination in the laboratory. Non-probability statistical tools conferred 42% (84/200, CI=35.37-48.93) prevalence of subclinical mastitis, 38.1% S. aureus (32/84, CI=28.45-48.79), 15.6% MRSA (5/32, CI=6.87-31.76), 46.9% haemolytic S. aureus (15/32, CI=30.87-63.56) and 34.4 % biofilm producing S. aureus (11/32, CI=20.41-51.69). Earthen floor type (OR=1.75, p=0.0996), poor drainage system (OR=7.33, p=0.002), pond as source of drinking water (OR=2.05, p=0.179), stall feeding (OR=7.27, p<0.001), 4-6 years of age of goat (OR=4.2, p=0.0874), and teat injury (OR=13.74, p<0.001) were potential risk factors for subclinical mastitis. The in-vitro findings of current study revealed 100% sensitivity of S. aureus against gentamicin, oxytetracycline, amoxicillin, and linezolid while 80% of biofilm negative S. aureus (nbpSA) showed sensitivity against amoxicillin+clavulanic acid. None of the isolate from bpSA and nbpSA was resistant against linezolid, gentamicin, and oxytetracycline in this study. bpSA were highly resistant against amoxicillin and vancomycin.The study found higher prevalence of pathogenic strains of S. aureus, higher number of potential risk factors, and diversified responses to antibiotic.
 

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Pakistan Journal of Zoology

August

Vol. 53, Iss. 4, Pages 1201-1601

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