Salmon Gonadotropin-Releasing Hormone Analogue (sGnRHa) as a Potent Ovulation Inducer for Artificial Insemination in Rabbit
Salmon Gonadotropin-Releasing Hormone Analogue (sGnRHa) as a Potent Ovulation Inducer for Artificial Insemination in Rabbit
Olufisayo Bamidele* and Safiriyu Idowu Ola
ABSTRACT
This study evaluated the fertility of non-lactating rabbit does artificially induced with Salmon Gonadotropin-releasing hormone analogue (sGnRHa). Forty multiparous rabbit does were randomly divided into four groups of ten does each in a Completely Randomized Design (CRD). The does in the control were naturally mated; the does in the other groups were at the moment of Artificial Insemination (AI) intramuscularly injected with 5µg/kgBW, 10µ/kg/BW and 15µg/kgBW of sGnRHa in Ovaprim®, respectively. Blood samples were collected pre injection and at 60 and 120 minutes post insemination. Data recorded were Plasma LH levels, kindling rate, total kit born, total born alive, still born, weight of kits at birth and gestation period (days) and were analyzed using Analysis of variance of the General Linear Model procedure and t-test where appropriate. The plasma LH for the AI groups peaked at 60mins while it peaked at 120mins for the control. Kindling rates were 10%, 10%, 60% and 50% respectively for the groups. There was no significant difference (p>0.05, t-test) between the litter size and total live kits obtained for 10µg and 15µg groups. Kit weight at birth showed no statistical difference (p>0.05, ANOVA) among all the groups. There was no still born in the control and the 5µg group. The gestation period (days) for the control and 5µg group were 32.00±0.00 while the gestation period (days) between the 10µg and 15µg groups was not statistically different (p>0.05, t-test). It was concluded that sGnRHa could be a potent substance for inducing ovulation in low sexually receptive does submitted for AI.
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