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Experimental studies on the efficacy of combined inactivated Infectious coryza and infectious bronchitis vaccine in chicken

Experimental studies on the efficacy of combined inactivated Infectious coryza and infectious bronchitis vaccine in chicken

Afaf. A. Khedr*, Ekram Salama*, A. A. Samy**, Abdella, Y.A.*** and Suzan. K. Tolba***

* Central Lab. for Evaluation of Vet. Biologics, Abbasia, Cairo.
** National Research Center, Giza, Egypt
*** Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo.

ABSTRACT

Two combined inactivated oil adjuvant vaccines against infectious coryza and infectious bronchitis diseases were prepared, one adjuvanted with mineral oil and the other with Montanide ISA-70. Evaluation of both vaccines were done by challenge test, haemagglutination inhibition test, ELISA test for Avibacterium Paragallinarum, and ELISA test, indirect haemagglutination inhibition test and ciliostasis score for infectious Bronchitis virus (IBV). The immune response for the antigenic component of both vaccines show high titre of antibodies, but Montanide ISA-70 adjuvanted vaccine was superior as shown in mean antibody titers against Avibacterium paragallinarum serovar A, B and C and infectious bronchitis. In addition, Montanide ISA-70 is lesser in viscosity that makes it easily injected, so, it is better to use for control of infectious coryza and infectious bronchitis diseases in chickens.

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Journal of Virological Sciences

July

Vol. 3, Iss. 1

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