Antimicrobial activities of Methanolic and Aqueous extracts of Nigella Sativa and Ficus Carica
Antimicrobial activities of Methanolic and Aqueous extracts of Nigella Sativa and Ficus Carica
SHAHZADA NADEEM ABBAS1, KIRAN JAMSHED1, SARAH BUSHRA NASIR2,
SAMAN SHAHID3 & NASIR MAHMOOD4
ABSTRACT
New antimicrobials agents are needed to combat drugs resistance in microorganisms. The aqueous and methanolic extracts of Nigella sativa and Ficus carica seeds were used against bacteria (Staphylococcus aureus and Escherichia coli) and Fungi (Candida albicans, Fusarium oxysporium and Microsporum canis) to check their antimicrobial activities. For antibacterial assays, the volume and concentrations of extracts used were 0.5 μl (62.5 μg), 1 μl (125 μg), 5 μl (625 μg) and 10 μl (1250 μg). Specific laboratory protocols of antibacterial and antifungal assays were adopted. Absorbance of the broth bacterial culture was measured after 72 hours by using photo spectrometer at the wavelength of 600 nm (A600). For antifungal assays, the volume and concentrations of extracts used were 2 μl (250μg), 4μl (500μg), 8 μl (1000 μg) and 10 μl (1250 μg). The methanolic extract of Nigella sativa (1μl, 125μg) and water extract of Ficus carica (1μl, 125μg) were found most effective against E. coli (A600 0.003) whereas, water extract of Ficus carica (1μl, 125μg) was found most effective against Staphylococcus aureus (A600 0.003). Nigella sativa methanolic extract (8 μl, 1000μg) was found most effective against Candida albicans and Fusarium oxysporum with inhibited fungal growth zone diameter of 15mm, while Ficus carica methanolic extract (10 μl, 1250 μg) was found most effective against Fusarium oxysporum and Microsporum canis with diameter of inhibited fungal growth zone 19 mm and 21 mm, respectively. These experiments showed that these extracts can be used as antibacterial agents.
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