Shenhong Buxue Granules Improve the Vascular Endothelial Injury and Hematopoietic Function in Rats with Aplastic Anemia
Shenhong Buxue Granules Improve the Vascular Endothelial Injury and Hematopoietic Function in Rats with Aplastic Anemia
Xiaowei Huang, Yajie Wang, Jia Zhou, Junxiu Liu, Zhe Lin and Ruili Li*
ABSTRACT
Shenhong Buxue granule (SBG) is a traditional Chinese medicine compound recipe for treating blood deficiency syndrome. To investigate its effects on the hematopoietic function, aplastic anemia (AA) rat model was established by injection of cyclophosphamide (CTX) intraperitoneally. 60 SD rats were randomly divided into 6 groups, control, model, positive drug (compound E-jiao slurry), SBG high-, medium- and low-dose (SBG-H, SBG-M, SBG-L) groups. After 21 days of intervention, the changes of thymus and spleen indexs were compared, the levels of white blood cell (WBC), red blood cell (RBC), hemoglobin (Hb), hematocrit (HCT) and platelet count (PLT) in peripheral blood were detected, and the changes of hemorheology were observed. Serum levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), nitric oxide (NO), superoxide dismutase (SOD), malondialdehyde (MDA), lactate dehydrogenase (LDH) and reduced glutathione peroxidase (GSH-Px) were detected by ELISA. The expression level of TLR4/NF-κB, and HandE staining was used to observe the renal tissue. In the results, compared with model group, the spleen index and whole blood viscosity was significantly decreased, while the thymus index, peripheral blood WBC, RBC, Hb, HCT, PLT levels, and NO, SOD, GSH-Px levels in serum were significantly increased. The levels of TNF-α, IL-6, MDA, LDH in serum were significantly decreased. The protein expression levels of TLR4 and Nuclear factor-κB (NF-κB) p65 in kidney were significantly decreased. HandE staining on renal showed that SBG groups had a certain protective effect on renal injury, especially the high-dose group. In conclusion, SBG may improve hematopoietic function and improve vascular endothelial injury in AA model rats by regulating TLR4/NF-κB signaling pathway.
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