Regulation of Collagen Metabolism in Keratinocyte-Fibroblast Organotypic Co-culture by Pressure
Regulation of Collagen Metabolism in Keratinocyte-Fibroblast Organotypic Co-culture by Pressure
Yang Liu1,2,3, Baimei Liu1,2,3, Chenchen Li1,2,3 and Meiwen An1,2,3,*
ABSTRACT
Hypertrophic scar (HS) is characterized by aberrant fibroblast phenotypes and excessive deposition of extracellular matrix. Pressure therapy is a common treatment to cure HS, which can regulate collagen synthesis and deposition. To investigate collagen metabolism in keratinocytes and fibroblasts co-culture under pressure, and study the role of IL-1α and MMP-3 in terms of regulating fibroblasts-keratinocytes collagen metabolism with pressure.Chitosan-gelatin scaffolds were fabricated by vacuum freeze drying. Keratinocytes and fibroblasts were seeding on scaffolds to form systems as: keratinocytes monoculture (KM), fibroblasts monoculture (FM), co-culture of keratinocytes and fibroblasts (KM&FM). Systems was treated with 3.4 kPa pressure for 1 day, systems without pressure was considered as control groups. The mRNA expression and protein secretion of supernatant were tested using Q-PCR and ELISA method, respectively. Co-culture KM&FM under 3.4 kPa pressure enhanced typeIcollagen and type III collagen mRNA expression and protein secretion from KM. It however reduced typeIcollagen and type III collagen mRNA expression and protein secretion from FM; it promoted mRNA expression and protein secretion of IL-1α from KM and enhanced expression of MMP-3 from FM. The MMP-3 mRNA expression and protein secretion from FM were respectively positively correlated with IL-1α from KM and negatively correlated with the expression of typeIcollagen and type III collagen of FM. The increased secretion of IL-1α from KM stimulated MMP-3 secretion from FM and promoted degradation of typeIcollagen and type III collagen from FM.
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February 2019
Vol. 51, Iss. 1, Pages 1-398
