Traditional and Molecular Detection of Cryptosporidium Parasite in Quails (Coturnix coturnix) in Baghdad Province, Iraq
Traditional and Molecular Detection of Cryptosporidium Parasite in Quails (Coturnix coturnix) in Baghdad Province, Iraq
Khitam J. Yahya*, Mohammed T. S. Al-Zubaidi
ABSTRACT
Cryptosporidiosis is a wildword disease among birds. Detection of cryptosporidiosis in quails required to be more sensitivity and specificity using molecular analysis and sequences. A total of 100 fecal samples were collected from quails in various parts of Baghdad/ Iraq from January to September, and they were then tested for the presence of Cryptosporidium spp. using molecular methods (PCR), followed by DNA sequencing. The overall infection rate was 37% (37/100) of the samples were Cryptosporidium-positive by using PCR. Three of five quails whose 18S gene was amplified by nested PCR and sequenced were found to belong to C. meleagridis in the sequence of this gene deposit on the website of the National Center for Biotechnology Information (NCBI) under accession number (HQ917077.1), while the others were found to be closely related to C. baileyi and had the accession number (MN410723.1). The finding suggests that Cryptosporidium parasites, which cause zoonotic disease transmission, are widespread among Baghdad quails.
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