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Isolation of Pseudomonas aeruginosa Phages from Wastewaters

Isolation of Pseudomonas aeruginosa Phages from Wastewaters

Stephen Chijioke Emencheta1, Chinelo Charity Eze1, Anthony Amaechi Attama2, Damian Ejike Agbo1 and Ebele Benedette Onuigbo1*

1Department of Pharmaceutical Microbiology and Biotechnology, University of Nigeria, Nsukka, 410001, Nsukka, Enugu State, Nigeria; 2Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka, 410001, Nsukka, Enugu State, Nigeria.

 
*Correspondence | Ebele Benedette Onuigbo, Department of Pharmaceutical Microbiology and Biotechnology, University of Nigeria, Nsukka, 410001, Nsukka, Enugu State, Nigeria; Email: ebele.onuigbo@unn.edu.ng

ABSTRACT

This research was designed to isolate and test the lytic action of a bacteriophage specific to Pseudomonas sp. Both Pseudomonas spp. and bacteriophages were isolated from residential wastewaters. The isolated Pseudomonas spp. were confirmed through biochemical tests. Antibiogram was done with nine (9) different antibiotics, including;aztreonam (ATM), chloramphenicol (CPL), gentamicin (GTN), tetracycline (TTE), sulphamethoxazole/trimethoprim (SXT), amoxicillin/clavulanic acid (AMC), meropenem (MEM), ciprofloxacin (CIP), ceftriaxone (CRO)). Plaque assay was done to determine lytic action of the bacteriophage on the lawns of the Pseudomonas sp. The host range of the isolated bacteriophages was also assessed. Cultural characteristics and biochemical tests confirmed the Pseudomonas aeruginosa isolates. The bacterial isolates were sensitive to only ciprofloxacin. The Multi Antibiotic Resistance Indexes (MARI) of the isolated strains were greater than 0.8. The formation of plaques (clear zone) on the lawn of Pseudomonas spp confirmed lytic action. The bacteriophage showed plaques on Pseudomonas sp. isolated from other sites also. The isolation of Pseudomonas aeruginosaphages from residential wastewaters is a promising molecular tool in combating the global antimicrobial resistance threat.

 

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Hosts and Viruses

June

Vol. 8, Iss. 3, Pages 1-20

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