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Pinocembrin Ameliorates Neuronal Cell Death by Regulating Autoimmune Signals

Pinocembrin Ameliorates Neuronal Cell Death by Regulating Autoimmune Signals

Hairul Islam M. Ibrahim1,2, Abdalla A. Sayed1,3, Abdel Naser A. Ahmed4,5 and Emad A. Ahmed*1,6

1Department of Biological Science, College of Science, King Faisal University, P.O. Box 400, Al-Ahsa, 31982, Saudi Arabia 
2Pondicherry Centre for Biological Sciences and Educational Trust, Kottakuppam, Pondicherry 605104, India
3Department of Zoology, Faculty of Science, Al-Minia University, Minia, Egypt 
4College of Agricultural and Food Science, King Faisal University, P.O. Box 400, Al-Ahsa, 31982, Saudi Arabia 
5Department of Animal Breeding, Faculty of Agriculture, Assiut University, Assiut, Egypt
6Laboratory of Molecular Physiology, Department of Zoology, Faculty of Science, Assiut University, Assiut, Egypt
 
* Corresponding author: eaahmed@kfu.edu.sa

ABSTRACT

Pinocembrin is a type of flavonoid present in honey and propolis. It has high antioxidant and neuroprotective effect. The present study was designed to evaluate the immunomodulatory properties of pinocembrin (PCB) and the anti-apoptosis effects using in vitro and in vivo mice splenocytes and experimental autoimmune encephalomyelitis (EAE) in C57BL/6j female mice, respectively. The reduction of clinical symptoms and disease index were evaluated by histochemical analysis. T-cell population and cytokines levels in myelin oligodendrocyte glyco-protein (MOG) challenged PCB treated cells were estimated using flow cytometry and ELISA kits. Apoptotic and neuronal markers were evaluated using quantitative real time PCR and protein blots. Results showed that PCB inhibited the infiltration of inflammatory cells and improved the myelin protective proteins. It also positively regulated the antioxidants and apoptotic markers including Caspase-3, TGF-β, SIRT-1, CCL-2 and MBP. Moreover, PCB modulated the levels of inflammatory mediators and TGF-β in splenic cells. The production of myelin basic protein (MBP) increased in PCB treated EAE mice. Taken together, the current finding revealed that, PCB suppressed EAE pathological checkpoints for demyelination and apoptosis in CNS and act as a therapeutic role for treatment of MS in upcoming prospects.
 

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Pakistan Journal of Zoology

October

Vol. 54, Iss. 5, Pages 2003-2500

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