In-Vitro Propagation for Conserving the Gems of the Hills Caralluma tuberculata to Rescue the Valuable Endangered Medicinally Important Plant
In-Vitro Propagation for Conserving the Gems of the Hills Caralluma tuberculata to Rescue the Valuable Endangered Medicinally Important Plant
Muhammad Taqi1, Shazia Erum2, Shamaila Rasheed2, Sadar Uddin Siddiqui2 and Shakeel Ahmad Jatoi2*
ABSTRACT
Overharvesting has posed pressure on Caralluma tuberculata plants resulting in the disappearance of its wild populations in different mountainous regions in Pakistan. A proficient way for large-scale and fast propagation of C. tuberculata through in-vitro organogenesis was carried out at in-vitro lab, Bio-resources Conservation Institute, NARC, Islamabad, Pakistan. Nodal-tips as explants were grown on MS salts enriched with diverse plant growth regulators including BAP, NAA, Kin and 2,4-D, alone as well as in combinations. The highest growth response for callusing 78.3% was observed in BAP (0.5mg/L) in combination with Kinetin (0.25mg/L) +NAA (2 mg/L) followed by 65% at 2 mg/L of 2,4-D. Maximum percentage of shooting (81.67%) with the maximum mean value for number of shoots (7.56) and shoot length (7.07) occurred when the fresh shoot tip of C. tuberculata were planted on to MS salts containing 2 mg/L of BAP, 0.5 mg/L of NAA and 0.25 mg/L of Kin. The new shoots were then inoculated on 1/2 strength MS salts fortified with NAA and IBA root induction. The highest rooting percentage (61.67%), as well as root length (3.4 cm) was obtained with 0.5mg/L of both IBA and NAA. The current study could be beneficial for providing the basis to restore wild populations of C. tuberculata using in-vitro raised mass propagated plants, and help conserve the endangered germs of hills.
To share on other social networks, click on any share button. What are these?
