Evaluating the Impact of Various LED Light Spectrums on Dendrobium officinale Tissue Culture Seedlings
Evaluating the Impact of Various LED Light Spectrums on Dendrobium officinale Tissue Culture Seedlings
Hafiz Ishtiaq Ahmad1, Jinlong Zhang1, Fuxun Luo1, Owais Iqbal2 and Yuying Wang1*
ABSTRACT
Light plays a crucial role in plant growth and development and extends mere illumination it’s a pivotal environmental factor shaping plant physiology and morphology. To gain a comprehensive understanding, an experiment was designed to investigate the impact of various light-emitting diode (LED) spectrums including red, blue, yellow, green, and their composite lights on the plant growth parameters, physiological characteristics, and antioxidant activity of Dendrobium officinale tissue culture seedlings. The fluorescent white light served as control. The tissue culture seedlings were grown in simple light and transplanted about 0.1 cm long into a solid culture medium. The plants were subjected to various LED spectra for a duration of 14 hours each day and continuous subsequent 100 days. The experiment was completely randomized design with nine treatments and each treatment have ten replications. The SPSS statistics software was used to analyses experimental data. In addition, the result of the current study revealed that the plant height, leaf length, leaf width and root number were significantly increased in the red, blue and white (RBW) composite treatment, while composite red, blue and yellow (RBY) subjected seedlings exhibited maximum leaf number, root length, and proliferation rate as compared to the others. Therefore, seedlings exposed to the light combination of red and blue (RB) exhibited the highest chlorophyll a and b, total chlorophyll content, catalase and root activity. The soluble sugar, soluble protein, superoxide dismutase and peroxidase levels increased significantly under compound red, blue and green (RBG) light conditions, while malondialdehyde content was highly decreased in the same treatment (RBG) as compared to control. The study indicated that the various light qualities could enhance the growth parameters of D. officinale seedlings by improving biochemical features and their enzymatic activities.
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