Molecular detection of Contagious Pustular Dermatitis Virus (CPDV)isolatedfrom infected Sheep and Goats inQaliubeya Governorate, Egypt
Molecular detection of Contagious Pustular Dermatitis Virus (CPDV)isolatedfrom infected Sheep and Goats inQaliubeya Governorate, Egypt
El-Habbaa, A.S.
ABSTRACT
Contagious Pustular Dermatitis Virus (CPDV) of genus parapoxvirus, subfamily
Chordopoxvirinae, familyPoxviridae, causesContagiousPustular Dermatitis in sheep and
goats worldwide and in Egypt. Forty samples of skin lesions from suspected 30 sheep
and 10 goats from different areas of Qaliubeya governorate were subjected
tovirusisolation on embryonated chicken eggs (ECE), antigen detection using agar gel
immune-diffusion test (AGIDT) and indirect-Immunofluorescence assay (IFA) and
molecular detection of viral DNA using PCR. Positive results were showed in 36 of
samples (28/30 of sheep samples and 8/10 of goat samples)upon isolation on ECE by the
3rd passage and 22 of samples (17/28 of sheep isolates and 5/8 of goat isolates) using
AGIDT and indirect-IFA. PCR detection showed positive results with 30 /40 of samples
before isolation (23/30 and 7/10 of sheep and goat samples, respectively) while it
showed positive results with 26 /36 of samples upon isolation (20/28 and 6/8 of sheep
and goat samples, respectively). It was concluded that AGIDT and indirect-IFA were
suitable methods for CPDV detection but PCR was most specific and sensitive
diagnostic tool for rapid detection of CPDV.
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December 2013
Vol. 10, Pages 1-171
