Indole-3-Carbinol of Broccoli Powder Can Decrease Estrogen Production in Male Rats
Indole-3-Carbinol of Broccoli Powder Can Decrease Estrogen Production in Male Rats
Reski Amalia1, Claude Mona Airin2, Pudji Astuti2*, Agung Budiyanto3
ABSTRACT
The increasing use of plants as a form of medicine presents fewer risks today. Broccoli (Brassica oleracea var. italica) has been shown to influence various physiological processes, including hormonal modulation. This study aimed to evaluate the effect of broccoli powder on estradiol (E2) levels in male rats. Over a period of 28 days, male rats were orally administered broccoli powder containing indole-3-carbinol (I3C). A total of 25 rats were divided into 5 groups (P1 to P5) and supplemented with either distilled water (negative control), letrozole (positive control), or broccoli powder at dosages of 0.117 g/kg BW, 0.234 g/kg BW, and 0.460 g/kg BW. Weekly blood samples and body weight measurements were taken over the 4-week period, and necropsy was performed in the final week to collect the testicles and brain. Testis and body weight were quantified, and E2 levels were measured by ELISA. Results showed that body weight was significantly higher (p<0.05) in groups P1 (192.32 ± 2.85g), P2 (199.96 ± 3.02g), and P3 (206.56 ± 2.96g) compared to groups P1 (179.29 ± 2.91g) and P2 (185.48 ± 2.75g). However, no significant differences (p>0.05) were observed in brain weight, testis weight, or testis length. The broccoli powder significantly affected E2 levels in male rats, with notable (p<0.05) effects in groups P4 and P5 during Week 4, showing values of 2.99 ± 0.49 pg/ml and 2.38 ± 0.48 pg/ml, respectively. These findings suggest that broccoli powder administration impacts body weight and deccreases E2 concentration in male rats, potentially benefiting reproductive success and function.
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