Post Thawed Quality of Tharparkar Bull Semen Extended in BioxcellTM and Tris-Based Egg Yolk Extenders Supplemented with Vitamin E (α-Tocopherol)
Post Thawed Quality of Tharparkar Bull Semen Extended in BioxcellTM and Tris-Based Egg Yolk Extenders Supplemented with Vitamin E (α-Tocopherol)
Abdullah Channo1,2*, Asmatullah Kaka1, Akeel Ahmed Memon1, Mool Chand Malhi3, Muhammad Bakhsh4, Qudratullah Kalwar5, Shakeel Ahmed Tunio6 and Muhammad Ibrahim Panhwar7
ABSTRACT
The study was designed to determine the effect of Vitamin E (α-tocopherol) supplimentation into BIOXcellTM and Tris based egg yolk extender on post-thawed quality of Tharparkar cattle bull semen. The research was carried out at Department of Animal Reproduction, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agricultural University Tandojam. In this study four fertile Tharparkar bulls (A, B, C and D) having age of 4-5 years were used. A total of 52 (n=13) ejaculates were collected with the help of artificial vagina, after the collection macroscopic (color, volume and pH) and microscopic (motility, morphology, viability, concentration and membrane integrity) parameters were observed. The samples having motility, morphology, viability and membrane integrity ≥70% were pooled and processed. Pooled semen samples were divided into four groups and diluted with Tris, Tris+Vitamin E(α-tocopherol) and BIOXcellTM, BIOXcellTM+Vitamin E (α-tocopherol). Post-thawed assessment of motility, morphology, viability and membrane integrity with BIOXcellTM+ Vitamin E(α-tocopherol) (58.31±0.86, 76.22±1.04, 71.27±1.44, 64.68±1.43) showed improved quality parameters as compared to Tris+ Vitamin E(α-Tocopherol) (48.68±0.68, 64.45±1.54, 66.22±1.75, 58.25±1.08), BIOXcellTM (45.72±0.53, 67.45±0.84, 64.72±1.67, 56.63±1.39) and Tris (43.54±0.49, 64.68±0.80, 65.18±1.33, 54.41±1.36). On the basis of in vitro results BIOXcellTM+Vitamin E(α-tocopherol) showed improved post-thawed quality parameters and it was used for artificial insemination. A total of 20 animals were synchronized at Bhens colony; Ghumanrabad with one injection prostaglandin (pgf2α) protocol and 13 animals showed estrus and were inseminated. Meanwhile, 8 were found pregnant through rectal palpation with a pregnancy rate of 61.53%.
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