The Genetic Characterization of Turkish Grey Cattle with Regard to UoG Cast, CAPN1 316 and CAPN1 4751 Markers
The Genetic Characterization of Turkish Grey Cattle with Regard to UoG Cast, CAPN1 316 and CAPN1 4751 Markers
Süleyman Kök1*, Sertaç Atalay1, Hasan Semih Eken2 and Mustafa Savaşçi3
ABSTRACT
Turkish Grey cattle (TGC) is one of Turkey’s native cattle breeds. TGC was a popular race to breeding within Thrace and Western Anatolia, but unfortunately they are under the threat of extinction recently. The application of gene specific molecular markers in genotyping and genetic identification is of essential significance for preserving genetic diversity. The aim of this study was to reveal the genotype profile of indigenous types for TGC breed. In order to characterize TGC population, two polymorphic loci in cattle Calpain gene (CAPN1) and one polymorphic locus in Calpastatin gene CAST were studied. In order to sort the allele variants (G/C), PCR products, the ones in CAST with RsaI enzyme, was cut using RFLP method. The allele variants (G/C) of CAPN1 316 and (C/T) of CAPN1 4751 were determined with ARMS-PCR method. This study was conducted to determine three SNPs which were related to meat quality in 130 purebred female and male samples of TGC breed. In TGC samples, C allele frequencies related to UoG-CAST, CAPN1 316 and CAPN1 4751 polymorphisms were found to be 0.6038, 0.1115 and 0.5654, respectively. CC genotype frequencies in the three polymorphic areas in TGC were calculated as 0.365, 0.012 and 0.320, respectively, while the distributions of the marker genotypes, except for CAPN1 4751 were found to be significant (P<0.05). The positive alleles of SNPs in Calpain and Calpastatin genes and all of their genotype combinations, were also found to be carried by TGC.
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