Intervening Strategies for Aflatoxin B1 in Cotton Seed Cake
Intervening Strategies for Aflatoxin B1 in Cotton Seed Cake
Deepesh Kumar Bhuptani1,2*, Atta Hussain Shah1, Muhammad Farooque Hassan2, Gul Bahar Khaskheli1, Zubair Ahmed Leghari3 and Qudratullah Kalwar2
ABSTRACT
Intake of low-quality feed specially aflatoxin contaminated cottonseed cake by dairy animals carry over the aflatoxins through milk and causing health problems (aflatoxicosis) to consumers. The present study was conducted with the objective to check the suitable binder through in-vitro experiments as aflatoxin level may be controlled in cottonseed cake. All the collected samples were transported aseptically to the Department of Animal Products Technology, Faculty of Animal Husbandry and Veterinary Science, Sindh Agriculture University Tandojam for analysis. In in-vitro study thirty (n=30) cottonseed cake samples with known level of aflatoxin B1 was extracted and treated with three binders Milbond, China Clay and Novasil. at three doses (T0 = recommended dose; T1 = < recommended dose; T2 = > recommended dose) to compare the adsorption rate. The China Clay and Novasil showed significant (p<0.05) effect as binders on adsorption ability of aflatoxin B1. Results revealed that the adsorption percent of T1 of Milbond (54.81%) was higher (p<0.05) than China Clay (47.09%) followed by Novasil (12.69%). Moreover, in case of the recommended doses (T0) by manufacturer the adsorption percent was 19.70, 67.88 and 12.41 % respectively for Milbond, China Clay and Novasil, respectively. Whereas higher dose treatments (T2) showed that adsorption percent was high (p<0.05) in Milbond (57.41%) followed by China Clay (47.45%) and Novasil (5.10%). It was concluded that China clay shown better adsorption at recommended dose than rest of the binders. Whereas, on lower and higher doses Milbond shown higher adsorption percent of AFB1 in cotton seed cake and in addition adsorption rate of Novasil increased (12.69%) with the dose reduction.
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