Comparative Evaluation of the Reaction of Indirect Hemagglutination, Enzyme Immunoassay and Serological Tests at the Diagnosis of Brucellosis in Cattle
Comparative Evaluation of the Reaction of Indirect Hemagglutination, Enzyme Immunoassay and Serological Tests at the Diagnosis of Brucellosis in Cattle
M.M. Mikailov1*, O. Yu. Chernykh2, E.A. Yanikova1, G.A. Nurlygayanova3, O.D. Sklyarov4
ABSTRACT
The results of comparative diagnostic studies of blood serum from cattle for brucellosis from farms with different epizootic situations, carried out in 2019 . The diagnostic efficacy of the indirect hemagglutination reaction (RNGA) was studied in comparison with the enzyme-linked immunosorbent assay (ELISA), agglutination reaction (RA), complement fixation reaction (CSC), rose-bengal test (RBP) and immunodiffusion reaction with O-PS antigen (RID with O -PS antigen). The specificity of these serological tests was confirmed by the negative results of studies of 40 blood serum samples from animals from a safe farm not vaccinated against brucellosis. Determination of the sensitivity of the tested diagnostic tools was carried out using 46 blood serum samples from cows immunized with a vaccine from the B. abortus 82 strain from a farm that is unfavorable for brucellosis. Studies have shown that RNGA is one of the most sensitive tests, which allows to identify in a dysfunctional economy a high percentage of animals with brucellosis (39.1% of those studied) in the early stages after infection and surpasses the results of most other serological methods. Data from comparative serological studies of cattle blood serum in RNGA, ELISA, RA, RSK, RBP and RID with O-PS, confirmed the specificity of the tests and high sensitivity of RNGA with brucellosis erythrocyte antigen
To share on other social networks, click on any share button. What are these?
