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The detection of Pregnancy Associated Glycoproteins in the blood Serum as an Indicator of Pregnancy in Bali Cattle

The detection of Pregnancy Associated Glycoproteins in the blood Serum as an Indicator of Pregnancy in Bali Cattle

I Ketut Puja1*, I Nyoman Sulabda2, Ni Nyoman Trinayani3, Ni Wayan Patmawati3, Made Rahayu Kusumadewi3, Putu Bulan Sasmita Dewi3, I Wayan Sukernayasa4, Anak Agung Gede Oka Dharmayudha5, Putu Devi Jayanti5, I Wayan Nico Fajar Gunawan5 

1Veterinary Genetics and Reproduction Technology Laboratory, Faculty of Veterinary Medicine, Udayana University, Bali, Indonesia; 2Laboratory of Veterinary Physiology, Faculty of Veterinary Medicine, Udayana University, Bali, Indonesia; 3Bali Cattle Breeding Center of BPTU-HPT Denpasar, Bali, Indonesia; 4Laboratory of Veterinary Reproduction, Faculty of Veterinary Medicine Udayana University, Bali, Indonesia; 5Laboratory of Veterinary Clinical Diagnostic, Clinical Pathology and Radiology, Faculty of Veterinary Medicine, Udayana University, Bali, Indonesia.

 *Correspondence | I Ketut Puja, Veterinary Genetics and Reproduction Technology Laboratory, Faculty of Veterinary Medicine, Udayana University, Bali, Indonesia; Email: asubali@unud.ac.id

ABSTRACT

Detection of early pregnancy in livestock is an important part of livestock reproductive management; therefore, choosing an accurate method for diagnosing early pregnancy is very important. This research aims to determine the potential utilization of pregnancy associated glycoproteins (PAG) circulating in the blood as a diagnostic tool for pregnancy in cows. A study was carried out on 37 Bali cows with previous experience giving birth. Blood samples were taken from the jugular vein on the 45th day after artificial insemination and stored at -20°C until PAG testing using the ELISA method. The sensitivity of the test for pregnancy detection was 0.33 ng/mL. As a control, samples from non-pregnant cows were used. The PAG concentration in pregnant Bali cows was 0.55 ng/mL, and in non-pregnant cows, it was 0.06 ng/mL. PAG concentration in pregnant cows was significantly different from that of cows that were not pregnant. The results of research on Bali cows showed that the accuracy of the PAG test in diagnosing pregnancy was 100%. Therefore, it was concluded that the circulating concentration of PAG is a suitable marker for determining pregnancy in Bali cattle. However, in order to better manage the reproductive system of Bali cows, further research is needed to increase the prediction value to a more acceptable starting point.
 

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Pakistan Journal of Zoology

June

Pakistan J. Zool., Vol. 56, Iss. 3, pp. 1001-1500

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