L-Carnitine Supplementation During In vitro Maturation of Egyptian Buffalo Oocytes Improved Embryo Yield and Decreased Blastocyst Apoptotic Rate
L-Carnitine Supplementation During In vitro Maturation of Egyptian Buffalo Oocytes Improved Embryo Yield and Decreased Blastocyst Apoptotic Rate
Shorouq Mohamed Omara1, Ibrahim Saad El-Shamaa1, Emad Abd-Elaziz Abd-Allah2, Assmaa Abd-Allah Fathy3, Essam-Eldin Tharwat4, Mohammed Hamdy Farouk5* and Ibrahim Mahmoud Abd El-Razek1*
ABSTRACT
The reproductive performance of Egyptian buffalo might be determined by genetic variants, but it is also affected by environmental factors. The possible action of L-carnitine (LC) on the maturation of Egyptian buffalo oocytes has not been determined yet. This study aims to estimate the effects of supplementing LC on the in-vitro fertilization (IVF) of Egyptian buffaloes. We evaluated blastocyst and apoptosis rates in order to substantiate the potential development of cumulus oocytes–complexes (COC) and determine the effect of LC in reducing the occurrence of apoptosis under in vitro maturation (IVM) conditions for buffalo blastocysts. The tissue culture media included a control culture medium and other three LC supplemented media (2, 3 or, 4 mM LC). All LC treatments had higher COCs percentages at the metaphase II (MII) compared to the control group. The 2 Mmol LC treatment had the highest percentage of oocytes maturation rate at metaphase ΙΙ (47%) compared to the control group (33.3%). The same treatment had a higher percentage of cleaved embryos (75.4% vs 64.9%) and blastocyst rates (43,4% vs. 31,5%, respectively). Furthermore, the same 2 Mmol LC treatment had a lower apoptotic rate of blastocyst compared to the control group (35,8% vs 58,3%). In conclusion, LC could increase the maturation and blastocyst rates of oocytes and reduce apoptosis in Egyptian buffalo embryos.
To share on other social networks, click on any share button. What are these?
