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Effect of Different Environmental Stresses on the Antioxidant Capability of Air-Breathing Loach, Paramisgurnus dabryanus (Sauvage, 1878)

Effect of Different Environmental Stresses on the Antioxidant Capability of Air-Breathing Loach, Paramisgurnus dabryanus (Sauvage, 1878)

Yaqiu Liu1, Zhijian Wang 2, Xinhui Li 1*, Jianrong Zhao2 and Weitao Chen 1

1Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou, China
2Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, Southwest University, Chongqing 400715, China

*      Corresponding author: lxhui01@aliyun.com

ABSTRACT

The effect of oxidative stress on gill and gut enzyme activity in Paramisgurnus dabryanus was studied under different breathing restraint treatments. A total of 150 healthy mature individuals (mean initial body weight = 16.1 ± 3.7 g and mean initial total fork length = 13.1 ± 1.2 cm) were selected for the experiment. The experimental specimens were separated into three modes: control group (n = 50) without any stress treatments; inhibited group (n = 50) with gut respiratory inhibition; and an air-exposed group (n = 50) with gill respiratory inhibition. After a 7 d acclimation for the inhibited and air-exposed groups, individuals were returned to the same conditions as the control group for a further 3 d acclimation. The catalase (CAT) and superoxide dismutase (SOD) activity in the gill and gut in P. dabryanus were examined in all three groups after the 7 d stress acclimation, 1 d later, and after a 3 d recovery. It was found that CAT and SOD activity in the gill, foregut, and midgut in the air-exposed group were much lower than in the control group. After recovery for 1 d, CAT activity in the gill and middle gut increased rapidly and was higher than in the inhibited and control groups. The SOD activity in the gill and gut also significantly increased, although there was no significant difference between the air-exposed and control groups. After a 3 d recovery, CAT and SOD activity in the gill and gut of P. dabryanus did not differ significantly between groups. There was a high viability of P. dabryanus under the air-exposed condition (in anaerobic conditions), while the level of oxidative stress in the gill, anterior, and middle gut clearly decreased. When recovering under normoxia conditions, the physical level of oxidative stress quickly returned to normal levels.
 

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Pakistan Journal of Zoology

June

Vol. 53, Iss. 3, Pages 801-1200

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