Morphological and Molecular Studies Nematode of the Species Parascaris equorum (Ascaridida)
Morphological and Molecular Studies Nematode of the Species Parascaris equorum (Ascaridida)
Sardorbek N. Turgunov, Oybek O. Amirov*, Erkinjon B. Shakarboev, Abdurakhim E. Kuchboev
ABSTRACT
This article presents the results of research on the morphometric and molecular characteristics of the nematode Parascaris equorum collected from horses of the Fergana Valley, Uzbekistan. The total length of the male Parascaris equorum nematode was 175.5±3.86 mm, and that of the female was 293.7±4.83 mm. For molecular genetic studies from the collected samples, genomic DNA was extracted using the head of male of P. equorum species. QIAamp DNA Mini Kit reagents (QIAGEN, Germany) were used for genomic DNA isolation. According to the results of molecular genetic study, the nucleotide sequence belonging to the ITS1-5.8S-ITS2 region of the ribosomal DNA of P. equorum species was analyzed, and it was found that 100% nucleotide similarity with P. equorum species (MH030605) and 95.85% similarity with the species P. univalens (MK209648) in the GenBank database (NCBI).
Keywords | Nematode, Horse, Helminth, Ribosomal DNA, ITS2, Parascaris equorum
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