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Mechanism of Antipyretic Effect of Saposhnikoviae Radix Based on Network Pharmacology and Experimental Verification

Mechanism of Antipyretic Effect of Saposhnikoviae Radix Based on Network Pharmacology and Experimental Verification

Taidong Wang1, Xiaowei Huang1, Jian Huang2, Guangfu Lv3 and Zhe Lin1*

1College of Pharmacy, Changchun University of Chinese Medicine, Changchun, 130117, China
2National Engineering Laboratory for Druggable Gene and ProteinScreening, Northeast Normal University, Changchun 130117, China
3Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun, 130117, China
 
* Corresponding author: linzhe_1966@163.com

ABSTRACT

The aim of this study was to explore the antipyretic role and mechanism of Saposhnikoviae Radix (SR). To achieve the objectives TCM-ingredient-target network and PPI network were constructed by TCMSP, TTD, DisGenet, OMIM databases and Cytoscape 3.7.2 software, and topology analysis was performed on the PPI network. KEGG signaling pathways were analyzed using DAVID database, and molecular docking verification was performed on the core targets after topology analysis. Subsequently, 24 rats were randomly divided into control group, model group, wild high-dose group and wild low-dose group, 6 rats in each group. Results of in vivo experiments showed that high-dose group (P<0.01) and low-dose group (P<0.01) significantly decreased body temperature in rats; high-dose group significantly decreased TNF-α, IL-1β and IL-6 levels (P<0.01), and low-dose group significantly decreased TNF-α and IL-6 levels (P<0.01); high-dose group significantly decreased BAX, caspase3, COX2 and TNF-α protein expression levels in hypothalamus (P<0.01 or P<0.05), and low-dose group significantly increased BCL2 protein expression levels (P<0.01). It was found that SR can significantly inhibit lipopolysaccharide-induced fever in rats with elevated body temperature, reduce serum TNF-α, IL-1β and IL-6 levels, reduce the hypothalamus COX2, caspase3 and Bax protein expression levels and improve BCL2 protein expression levels, play an antipyretic effect.

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Pakistan Journal of Zoology

February

Pakistan J. Zool., Vol. 56, Iss. 1, pp. 01-501

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