Protective Effects of Intestinal Trefoil Factor against Endotoxin-Induced Injury of Intestinal Mucosal Epithelial Cells in Piglets
Protective Effects of Intestinal Trefoil Factor against Endotoxin-Induced Injury of Intestinal Mucosal Epithelial Cells in Piglets
Zhenyu Chang1, Hui Zhang1, Khalid Mehmood1,2, Mujeeb Ur Rehman1, Xiaodan Yuan1, Zhaoyang Li1, Fazul Nabi1, Xiaoxing Wu1, Xinxin Tian1, Xueting Liu1, Jingen Xu3 and Donghai Zhou1,*
ABSTRACT
Individual intestinal epithelial cells can survive and maintain their activity for several hours in tissue samples. For this study piglets without colostrum feeding were selected. Primary cell culture and subculture was performed from the intestinal epithelial cells of piglets. Then the effects of intestinal trefoil factor (TFF3) in intestinal disease was explored. Porcine intestinal epithelial cells were treated with lipopolysaccharide (LPS) and recombinant human (rh) TFF3. Cell proliferation and TFF3 mRNA expression were examined by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assays and real-time quantitative PCR, respectively. The results showed that rhTFF3 effectively prevented the cell injury induced by LPS (P<0.05). A low concentration of LPS (10 µg/mL) down-regulated the expression of TFF3 mRNA, whereas a high concentration of LPS (100 µg/mL) up-regulated TFF3 mRNA expression. Furthermore, TFF3 mRNA expression was decreased by rhTFF3 treatment. These findings indicate that TFF3 attenuates LPS-induced cell damage, and TFF3 mRNA expression is influenced by LPS and rhTFF3 in intestinal mucous epithelial cells in piglets.
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December 2017
Vol. 49, Iss. 6, Pages 1937-2341
