Submit or Track your Manuscript LOG-IN

Chia Seeds Oil Inhibits Hepatic Resistance to Doxorubicin Via Suppressing Cyp3-A4 and Mrp-1 in Male Albino Rats

Chia Seeds Oil Inhibits Hepatic Resistance to Doxorubicin Via Suppressing Cyp3-A4 and Mrp-1 in Male Albino Rats

Shaimaa A.Tawfik2,3, EL S.T. Awad1*, Hoda O.Abu Bakr1, Amira M.Gamal-Eldeen4, Esmat Ashour2, Ismail M.Ahmed1  

1Department of Biochemistry and Molecular biology, Faculty of Veterinary Medicine, Cairo University; 2Biochemistry Department, National Research Centre, 33 El Buhouth St. Dokki 12622, Giza, Egypt; 3Cancer biology and Genetics Laboratory, Centre of Excellence for Advanced Sciences, National Research Centre, 33 El Buhouth St. Dokki 12622, Giza, Egypt; 4Clinical Laboratory Sciences Department, College of Applied Medical Sciences, Taif University, P.O.Box 11099, Taif21944, Saudi Arabia.

*Correspondence | El S. T Awad, Department of Biochemistry and Molecular biology, Faculty of Veterinary Medicine, Cairo University; Email: elsaeedthabet1234@gmail.com 

ABSTRACT

Background: The oil-seed of chia (Salvia hispanica L.), CSO, had been reported for many biological activities. Doxorubicin (DOX) is one of the active chemotherapies for hepatocellular carcinoma. DOX-resistance is the cause of its limited use. Objectives: This study aimed to explore the influence of CSO on DOX-induced resistance and antioxidant status. Methods: 40 male albino rats weighing 150-200g were divided equally into four groups 10/each. CSO (5% w/w) were administrated oral daily for 30 consecutive days. DOX treated groups were injected IP at a dose of 2.5 mg/kg trice weekly to be totally 15 mg/kg for two weeks. G1 Control, G2 CSO, G3 Doxorubicin, G4 CSO/DOX. Antioxidant activity, histopathological examination, immunohistochemical analysis of CYP3A4, MRP-1, and c-MYC proteins and expression of miRNAs were assayed. Results: Findings indicated that CSO treatment led to a suppression in hepatotoxicity, nephrotoxicity and cardiotoxicity induced by DOX in male albino rats evidenced by a significant enhancement in the activity of ALT, GGT, LDH, AST, also creatinine and uric acid. CSO induced antioxidant activity GSH, CAT and inhibited MDA. Histopathological examination in sections of liver, kidney and heart tissues were confirmed these findings. In liver tissues, DOX resulted in an observable induction in CYP3A4 and MRP-1, while CSO/DOX showed an inhibition in both proteins. c-MYC was dramatically decreased in DOX group, while CSO/DOX group restored cellular c-MYC. CSO/DOX group resulted in a remarkable inhibition in the tumor suppressor miRNA (let-7a) compared to DOX-induced expression. Moreover, miR-122, as negative regulator of DOX resistance, showed a dramatic induction in CSO/DOX group compared to DOX group. Conclusion, CSO is capable of effectively inhibit DOX resistance, induced hepatic, nephro and cardiac antioxidant status, inhibit lipid peroxidation, and restore hepatic function.
 

To share on other social networks, click on any share button. What are these?

Advances in Animal and Veterinary Sciences

June

Vol. 12, Iss. 6, pp. 994-1205

Featuring

Click here for more

Subscribe Today

Receive free updates on new articles, opportunities and benefits


Subscribe Unsubscribe