Efficacy Prediction of Autologous Platelet-Rich Plasma (PRP) against Sub-clinical Bubaline Mastitis
Efficacy Prediction of Autologous Platelet-Rich Plasma (PRP) against Sub-clinical Bubaline Mastitis
Qamar Ullah1,2, Muti-ur-Rehman Khan1*, Raheela Akhtar1 and Aftab Ahmad Anjum3
ABSTRACT
The current study was conducted on riverine-type buffaloes suffering from sub-clinical mastitis (SCM). The domestic water buffalo (Bubalus bubalis) provides milk, meat, hides, and draught power and gives a significant share to the agricultural economy of the globe. The river buffalo and the swamp buffalo are two distinct types of water buffalo that are present in different places of the world. River buffalo have been preferred for their superior ability of milk production. Mastitis is among the topmost health concerns of farm animals, causing massive financial damage to the agro-livestock sector globally. A total of 96 udder quarters/teats of buffaloes with SCM were arbitrarily allotted to three different groups, i.e., platelet-rich plasma (PRP) group (n=32), antibiotic group (n=32) and PRP plus antibiotic group (n=32). Whole blood was taken from the animal’s jugular vein, and PRP was prepared by double spin open method and infused intra-mammary to the same animal from which the blood was taken. An intra-mammary antibiotic was administered intra-mammary to the animals of the antibiotic group. PRP plus antibiotic was allocated to the animals of the combined associated treatment group. Platelets significantly increased (p<0.05) and decreased WBCs and RBCs values in PRP. A significant decrease (p<0.05) was observed in the somatic cell count (SCC) of milk of animals treated with PRP. The use of PRP revealed 9.67 times higher chances of recovery compared to antibiotic alone, whereas the combination of PRP plus antibiotic was 4.33 times more effective than antibiotic only. Moreover, PRP was 55% more productive than the combination of PRP plus antibiotics. The area under the curve calculated by receiver operating characteristic (ROC) analysis predicted an acceptable area under the curve (0.73) regarding the efficacy of autologous PRP alternative to the antibiotic in sub-clinical bubaline mastitis. A significant (p<0.05) difference was observed in the mean rank difference among the three treatment groups through the non-parametric Kruskal-Wallis test. It was concluded that PRP plays a crucial role in tissue repair and regeneration of the injured mammary gland of buffaloes.
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