Submit or Track your Manuscript LOG-IN

FliI Role in Flagellar Assembly of Salmonella ΔfliI Mutant Strain Determines Motility and Biofilm Formation

FliI Role in Flagellar Assembly of Salmonella ΔfliI Mutant Strain Determines Motility and Biofilm Formation

Iram Liaqat1,*, Safdar Ali Mirza2, Sumera Sajjad3, Shaukat Ali1,*, Muhammad Faiz Qamar4 and Ikram Ul Haq5

1Department of Zoology, Govt. College University, Lahore-54000
2Department of Botany, Govt. College University, Lahore-54000
3Department of Zoology, Lahore College for Women University, Lahore
4Department of Pathobiology, College of Veterinary and Animal Sciences, Sub-campus Jhang
5Institute of Industrial Biotechnology, Govt. College University, Lahore-54000

*      Corresponding author: iramliaq@hotmail.com

ABSTRACT

Biofilms formation is a serious problem in both clinical and environmental settings. Various gram negative bacteria exhibit biofilm formation mediated by flagellum-mediated motility. Type III protein secretion systems of several gram-negative bacterial pathogens use flagella to invade foreign surfaces, host tissues and substrates. Flagellar biosynthesis and function in Salmonella typhimurium isregulated by >50 genes. Bioinformatics analysis of flagellar assembly in S. typhimurium identified several conserved structural elements. In this study, FliI a flagellar protein required for flagellar assembly and involved in a specialized protein export pathway was cloned and overexpressed. ΔfliI mutant Salmonella strain was used to transform fliI overproducing plasmid pTrc99A by electroporation. Using vital dyes (Alexaflour 488), visualization of motility was observed in wild type, Δ fliI mutant and fliI complemented strainwhich was further assessed by biofilm formation ability. Swimming, swarming motility alongwith significantly reduced biofilm formation was observed in Δ fliI mutant compared to wild type and fliI complemented strains. This study will extend initial evidence that FliI plays important role in flagellar export system and flagellum-mediated rotation is criticalfor swimming, swarming motility and biofilm formation. The flagellar basal body has an ancient and evolutionarily conserved macromolecular assembly and known architecture making it an ideal drug target. The knowledge obtained will help to elucidate mechanism and design principles necessary to understand protein secretion systems.
 

To share on other social networks, click on any share button. What are these?

Pakistan Journal of Zoology

December

Vol. 53, Iss. 6, Pages 2001-2521

Featuring

Click here for more

Subscribe Today

Receive free updates on new articles, opportunities and benefits


Subscribe Unsubscribe