Parasitological and Molecular Survey on Theileriosis of Sheep and Goats and the Related Risk Factors in Musa Pak Shaheed Town, Multan, Pakistan
Muhammad Riaz1*, Zahida Tasawar1, Muhammad Zaka Ullah2 and Zawar Hussain3
ABSTRACT
The current survey was designed to study the infections of Theileria spp. (Theileria lestoquardi and T. ovis) in small ruminants through PCR and microscopy in Musa Pak Shaheed, Multan district, Pakistan amidst 2013. A total of 200 small ruminants (93 sheep and 107 goats) without any apparent signs of theileriosis were examined to diagnose Theileria piroplasms. During sampling, blood was amassed by puncturing the jugular vein in eppendorf comprising EDTA as anticoagulant coated tubes for the detection of Theileria piroplasms. DNA was extracted from collected samples and subjected to PCR amplification to determine ovine and caprine theileriosis. Through a questionnaire, the data regarding animals as well as herds characteristics were gathered to define the risk factors favors the spread of Theileria spp. infection. 24% and 8.5% Theileria infection identified through PCR and microscopic examination during present investigation. The PCR detected 14.5% and 9.5% Theileria piroplasms infection in sheep and goats, respectively. Among Theileria species, PCR identified the incidence of T. lestoquardi and T. ovis was 16.5% and 5% respectively while 6% blood samples revealed mixed infection of both species in overall small ruminants. The infection rate of T. lestoquardi was higher (9.5% and 7%) than T. ovis (3% and 2%) in sheep and goats respectively. Statistically significant correlation revealed between theileriosis and animal species, different age groups, presence of ticks, herd size and herd composition in small ruminants. The outcomes of the current survey ratified PCR amplification is discernible, specific and sensitive assay for diagnosis of ovine and caprine theileriosis.
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