Kirana D.D. Adiputra1*, Tulus Maulana3 , Ekayanti M. Kaiin3, H. Hasbi2, Herry Sonjaya2
Kirana D.D. Adiputra1*, Tulus Maulana3 , Ekayanti M. Kaiin3, H. Hasbi2, Herry Sonjaya2
Kirana D.D. Adiputra1*, Tulus Maulana3 , Ekayanti M. Kaiin3, H. Hasbi2, Herry Sonjaya2
ABSTRACT
This study was conducted to determine the quality fresh and frozen semen of Bali and Simmental bull in the Technical Implementation Unit of Regional Artificial Insemination Center at Pucak, South Sulawesi. Two Bali bulls and two Simmental bulls aged 5-6 were used. The parameters observed in the fresh semen were volume, pH, colour, motility, viability, abnormality, intact plasma membrane, and concentration. Meanwhile, parameters observed in frozen semen were motility, viability, abnormality, intact plasma membrane, acrosome status, DNA integrity, and movement patterns. Viability and abnormality parameters were stained with eosin-nigrosine, intact plasma membrane was stained with Hypo-osmotic (HOS) solution, acrosome status was stained with peanut agglutinin (PNA), and DNA integrity was stained with acridine orange (AO). The results showed that the volume, pH, motility, abnormality, intact plasma membrane, and concentration of fresh semen of Bali and Simmental bull were not significantly different (P>0.05). Likewise, the frozen semen of Bali and Simmental breed did not show any significant differences (P>0.05) in terms of motility, acrosome status, intact plasma membrane, DNA integrity, and movement patterns, but the viability of Bali bull was significantly higher than that of Simmental bull and the abnormality of Simmental bull was significantly higher than that of Bali bull. Based on the results, it can be concluded both Bali and Simmental bull has the same quality of fresh semen, but Bali bull had better viability and abnormality compared to those Simmental breed of frozen semen.
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