Antinociceptive and Cytotoxic Effect of Extracts of Croton bonplandianus Leaves
Antinociceptive and Cytotoxic Effect of Extracts of Croton bonplandianus Leaves
Hina Yasin1,2*, Shaukat Mahmud2, Hina Abrar1, Kaneez Fatima3, Rabia Bushra1 and Saima Zahid2
ABSTRACT
Role of phytochemicals and plant based drugs in curtailment of diseases has already been established. Croton bonplandianus plant has found to be popular in the healthcare system for treatment of various complaints. In the present study, plant leaves were screened for analgesic and cytotoxic activities using animal models for the safe and effective utilization of plant material. The leaves were soaked in methanol and successively fractionated using n-hexane, chloroform, ethyl acetate and water. Mentioned biological activities were determined in different groups of wistar strain mice by administering various leaves extracts in differential dose pattern (81, 54 and 27 mg/kg). Cytotoxic activity was assessed by the Brine shrimp bioassay method. Results showed that extracts significantly responded as analgesic drug comparable to standard drug using different methods of evaluation for analgesic activity. Brine shrimp bioassay in different leaves extracts also showed similar effects likewise to the standard drug vincristine sulphate. All extracts exhibited marked cytotoxic activity as well against the Artemia salina (brine shrimp eggs). Based on the findings, it is concluded that the leaves extracts of the C. bonplandianus have potent analgesic and cytotoxic effects but in higher doses only. However; phytochemical screening and isolation of active constituents will need to be explored further to endorse the utilization of the Croton plant clinically for various therapeutic activities.
To share on other social networks, click on any share button. What are these?
