Effect of Fasudil Hydrochloride and H2 on the Post-Thaw Viability of Cryopreserved Porcine Adipose-Derived Stem Cells
Effect of Fasudil Hydrochloride and H2 on the Post-Thaw Viability of Cryopreserved Porcine Adipose-Derived Stem Cells
Maoying Zhu, Yuntao Ji, Xiaoyu Wang, Junying Pu and Changqing Qu*
ABSTRACT
The present study is to explore the effect of fasudil hydrochloride and H2 on the post-thaw viability of cryopreserved porcine adipose-derived stem cells. Four different combinations of cryoprotectants with and without hydrogen gas (H) (purified H2 was dissolved into normal cryopreservation solution for 2 hrs under 0.6 MPa were tested including the following groups: control (CK), 100µm GSH, 10µm fasudil hydrochloride (FH) and a combination of the two (GSH + FH). A solution of 10% (v/v) Me2SO + 20% FBS was used as the standard cryopreservation solution. After 2 months, MTT assay showed significant differences in the proportion of adherent viable cells in the FH group and GSH group compared with the control group (p < 0.05), and the FH+H group had a more beneficial effect on post-thaw survival of cryopreserved ADSCs compared with the GSH+H and GSH+FH+H groups. The use of FH and H in long-term storage has the potential to be helpful in commercial and clinical application of ADSCs.
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August 2017
Vol. 49, Iss. 4, Pages 1151-1546
