Relationship of Protein Profile in Blood and Seminal Plasma with Semen Quality of Egyptian Buffalo Bulls
Relationship of Protein Profile in Blood and Seminal Plasma with Semen Quality of Egyptian Buffalo Bulls
Hamdy Abdala Elnagar*, Wael Mohamed Wafa, Moataz Ibrahim Badwy, Abdelaziz Mustafa Sakr
ABSTRACT
The current study aimed to determine the possible relationship between blood plasma (BP) proteins and seminal plasma (SP) proteins in low and high fertile buffalo-bulls. Also, the relationship between protein profile in BP of adult bulls and bull calves were studied. Blood samples were taken from 3 calves (128.33±7.58 kg and ageing 6 mo.) and 10 bulls (400±37.5 kg and 24-25 months of age) and semen samples from bulls. Semen was collected once a week from all bulls which were divided according to semen quality to high and low fertile bulls (5 for each). Reaction time and semen characteristics were determined. Blood from bulls and calves, and seminal plasma of bulls were separated by centrifugation, and protein analysis was performed by using SDS-polyacrylamide gel electrophoresis. Results showed the presence of the molecular weight of proteins of 71, 52 and 31 kDa in BP and SP; 63, 33, and 19 kDa-proteins in SP, and 28 - 30 kDa-proteins in BP is in association with bull fertility (high semen quality). Also, 48, 18 and 16 kDa-proteins were present in the BP and SP of low fertile bulls. Proteins with 16, 30, 34, 35, 36, 37, 63, 64, 175, and 316 kDa were observed in the BP of bull calves. Proteins of BP and SP are related to bull fertility. Determination of protein profile in BP may be considered as a strategy for selection of semen quality of buffalo-bulls used for natural or artificial insemination. There is a relationship between protein profile in BP of buffalo-bulls and buffalo-bull calves which may suggest an early prediction of semen quality in bull calves in future studies.
Keywords | Buffalo-bulls, Blood, Semen, Protein, Fertility.
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