Characterization of Stromal Vascular Fraction Harvested from Inguinal Fat of Rabbit for Healing of Full Thickness Burn Wound
Characterization of Stromal Vascular Fraction Harvested from Inguinal Fat of Rabbit for Healing of Full Thickness Burn Wound
Abdul Aziz1,2, Hamad Bin Rashid1*, Muhammad Arif Khan1, Asim Khalid Mahmood1, Ayesha Hassan1, Hamid Akbar1, Sadaf Imran1, Naveed Hussain1, Muhammad Umar1, Muhammad Asif1, Sajjad Javaid1 and Mamoona Chaudhry3
ABSTRACT
Stromal vascular fraction (SVF) has become a promising candidate for regenerative therapy. SVF is preferred over culture-expanded stem cells due to its quick availability for clinical use and easy preparation. Burn wounds are a serious problem faced by veterinary clinicians in various animals. In this regard, the study was planned for the preparation and characterization of SVF from the adipose tissue of rabbits. Twelve healthy rabbits having body weight of 1000-1500g and of the same age were included in this study. SVF was prepared from bilateral inguinal fat that was harvested under general anesthesia. The harvested fat was enzymatically digested with 0.1% collagenase-I. It was then centrifuged and the SVF pellet was collected. The pellet was characterized through total viable cell count, cell per gram and percentage of viability by using Neubauer’s chamber. The average cell yield per microliter was 2.992±1.527 x 104 cells/µl. SVF per gram of adipose tissues was 2.992±1.527 x 106 cells/g, percentage of viability was 97.98± 0.31%, while non-viable cell count was 2.02± 0.31%. It is concluded that enzymatic digestion is an easy technique requiring less time for SVF isolation and gives better cell yield per gram. SVF preparation process has advantages of minimum cell contamination and processing time. Hence, it is a cost effective and alternate procedure for developing countries like Pakistan.
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