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Stability of Salmonella typhimurium Bacteriophage to Some Physical and Chemical Factors

Stability of Salmonella typhimurium Bacteriophage to Some Physical and Chemical Factors

Abeer A. Feisall; B.A. Othman2; Kh.A. El-Dougdoug2 and H.S. Shalaby1

1-Biotechnology Department, Higher Institute for Agriculture Co-Operation, Shoubra ELKheima, Cairo, Egypt.
2-Agriculture Microbiology Department, Faculty of Agriculture, Ain Shams University, Shoubra EL Kheima, Cairo, Egypt.

ABSTRACT

A lytic bacteriophage specific for Salmonellatyphimurium was isolated from
sewage water and designated St-1. The isolated phage formed identical clear
circular single plaques, without halo of 3 mm in diameter. Electron microscopy of
Salmonella bacteriophage particles (St-1) indicated that the phage particle has an
isometric head of about 69.6 nm in diameter and non contractile tail of 243.5 nm in
length and 17.4 nm in width. The Salmonella bacteriophage (St-1) has a narrow
host range since, it was infectious to Salmonella typhimrium ATCC25566 and S.
typhimurium MM11 among the eight bacterial isolates tested belonging to family
enterobacteriacae. The thermal inactivation point of the isolated phage was found to
be 76 ºC. Salmonella bacteriophage (St-1) survived for 7 days at 4, 25, 37, 42 and -
20 ºC. The virus lost its ability to lyse salmonella cells at pH 4, 5, 6, 10, 11 and 12
while it was infectious only at pH 7, 8 and 9.The virus lost its infectivity after
exposure to UV for 50 min. at distance of 53 and 70 cm from the UV source. The
bacteriophage (St-1) was inactivated in presence of NaCl at concentration higher
than 15%; sodium benzoate at concentration of 0.5%; potassium sorbate at
concentration of 1.0 % and citric acid at concentration of 1.0% as well as in
presence of 5% of either Sodium hypochlorite or SDS. The phage DNA was
resistant to digestion with PvuI, BamHI, XhoI and EcoRI. The genome size of the
phage was estimated to be 18 kbp.
 

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Journal of Virological Sciences

July

Vol. 8, Pages 1-27

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