Effect of Inorganic Selenium Supplementation on Gut Morphology and Growth Performance in Broilers under Stress Conditions in Khyber Pakhtunkhwa, Pakistan
Effect of Inorganic Selenium Supplementation on Gut Morphology and Growth Performance in Broilers under Stress Conditions in Khyber Pakhtunkhwa, Pakistan
Shafqat Ullah1, Asad Ullah2*, Imad Khan2, Rafiq Ullah1, Raheela Taj3, Fatima Syed3, Shumaila Gul4, Faiza Khan5, Ibad Ullah Jan6, Muneeb Islam7 and Sumaira8
ABSTRACT
Poultry industry is confronted to different hurdles which are blamed for the decline in the nutrients supplements for humans. One of the hurdles is the oxidative stress, which is responsible for the reduction of gut morphology and growth performance in the poultry birds. The present study was performed to explore the impact of inorganic selenium (sodium selenite). Day old chicks were kept in a control environment. At day 7, five groups were made with four replicas in each group based on the diet being offered. Group A, the positive control group fed with normal basal diet group B was termed as negative control group offered basal diet + dexamethasone and group C, D and E was offered 0.2mg/kg, 0.3mg/kg, and 0.4mg/kg of basal diet, respectively. The trial lasted for 35-days and on the last day two chickens from each replicate were slaughtered (a cumulatively eight chickens from each group). Samples from different regions (duodenum, jejunum, and ileum) of the small intestine were collected and kept in the fixatives. Slices from each part were stained with H and E stain to make it fit for microscopy. Picture were taken with different magnifications, i.e., 4X, 10X and 40X and histo-morphometry was done through Pixel-Pro (Labomed America Inc). The statistical analysis was through IBM SPSS (Statistics.V22.X86). The present study concluded that selenium improves the intestinal morphology i.e. villus height, villus width, villus surface area showed better growth(P≤0.05) in the selenium supplemented groups. The FCR initially did not differ (P≥0.05) among the groups but toward the end it was comparatively better (P≤0.05) in selenium supplemented groups. The relative body weight of different organs i-e liver, gizzard, heart, pancreas, and proventriculus etc. showed significant (P≤0.05) results in each of selenium-supplemented groups. Overall, 0.3 mg/kg (Basal diet) of selenium showed better results with respect to the positive control group.
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