Macroscopic Effect of Small Intestine Submucosa Hydrogel-Silver Nanoparticles Composite on Healing of Infected Wounds
Macroscopic Effect of Small Intestine Submucosa Hydrogel-Silver Nanoparticles Composite on Healing of Infected Wounds
Saddam Hummadi1, Nadia Al-Falahi2*
ABSTRACT
Treatment of infected wounds is one of the common challenges in veterinary practice. This study highlights the synthesis and use of hydrogel derived from small intestine submucosa (SIS) and AgNPs composite for accelerate the healing of infected wounds and improve cosmetic outcomes. A 5% w/v SIS hydrogel was prepared and formulated with 100 µg/ml AgNPs to evaluate its effect on healing of infected wounds in rabbit. Forty eight adult rabbits aged 8-12 months, weighing 1.5-2.5 kg, were divided randomly into three equal groups (n=16) after inducing infected wounds. In control group (GI), the infected wounds were managed by rinsing with normal saline after debridement and bandaging without any topical application. In SIS hydrogel group (GII), the infected wounds were treated by application of SIS hydrogel after wound management. In SIS hydrogel-AgNPs composite group (GIII), the infected wounds were treated by application of composition of SIS hydrogel and AgNPs after wound management. The wound healing was assessed clinically macroscopically by measurement of wound contraction at days (0, 3, 6, 9, 12, 15, 18, 21, 24, 27 and 30) post treatment in addition to macroscopic finding images (3, 7, 14 and 30 days) post treatment to monitoring the changing in wound bed. Such investigation indicates that the percentage of wounds closure were significantly increased (P≤0.05) in GII and GIII as compared to GI from day six extended to day thirty, while the significant increase in wounds closure in GIII were began from day 15th until day 24th post treatment and completely closed at day 27th without scar formation, in contrast with wounds area of GII which was nearly closed at periods of day 30th, while the wounds of GI exhibited incomplete closure at the same period. In conclusion, the composite bioscaffold integrates the properties of AgNPs with those of SIS hydrogel, providing a synergistic effect for wound healing improvement and showed the best outcome in healing of infected wound.
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