Influence of Administration and Withdrawal of Soy Phytoestrogens on Immune Perturbations of Ovariectomized Females Wistar Rats
Influence of Administration and Withdrawal of Soy Phytoestrogens on Immune Perturbations of Ovariectomized Females Wistar Rats
Shaza N. Alkhatib1, Rasha M. Ghoneim2, Hend M. Tag2,3, Hekmat M. Tantawy2, Heba M.A. Abdelrazek4*
ABSTRACT
Soy phytoestrogens play substantial role in estrogen replacement. The present research explored weather soy phytoestrogens mitigate cell mediated immunity perturbations caused by ovariectomy besides, the influence of 1 month of their dietary withdrawal on the same parameters. Therefore, forty-two female Albino rats were subjected to ovariectomy then divided into six groups. Experiment I: 3 groups (7 each); group A, served as control, received based casein diet, group B, fed low dietary soy (6.6%) for one month, and group C, fed high dietary soy (26.41%) for one month. Experiment II: Group D, control group (WC), rats were given based casein diet for two months, group E withdrawal low (WL) and group F withdrawal high (WH), received low (6.6%) and high soy (26.41%) for one month then transferred to a casein-based diet for another month. Body weight, feed intake, leukocytes count, lymphocytes transformation (LTT), tumor necrosis factor-alpha (TNF-α) and interleukins were assayed. Moreover, resistin, nitric oxide (NO), C-reactive protein (CRP), antinuclear antibodies (ANA) levels and cyclooxygenase-2 (COX-2) were estimated. Soy phytoestrogens diminished body weight gain (P<0.05), promoted total leukocytes count (P<0.05), abridged (P<0.05) the levels of interleukins, NO, CRP, resisting, ANA and COX-2 than control. Withdrawal of phytoestrogens in experiment II still keeping the reduced (P<0.05) interleukins, resisting, CRP, ANA. Dietary soy phytoestrogens mitigated estrogen depletion immunological perturbations in ovariectomized females. This effect could not be reversed even after one month from their withdrawal from diet due to the persistence of their immune modulatory effects. The soy phytoestrogens inclusion into diet ameliorates cellular immune response due to estrogen depletion. The ubiquitousness of phytoestrogens in the human or animal diets and fluctuation or alteration of their intakes or withdrawal propose their feature to human or animal health especially immune response that could be influenced on the rat study model and authorize further research.
Keywords: Cytokines, Inflammatory Markers, Phytoestrogens, Soy, Withdrawal
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October 2024
Vol. 12, Sp. Iss. 1
