Molecular Identification and Genetic Diversity of Notopterus notopterus (Pallas, 1769) Using Mitochondrial COI Gene and RAPD Markers in Riverine Water of Pakistan
Molecular Identification and Genetic Diversity of Notopterus notopterus (Pallas, 1769) Using Mitochondrial COI Gene and RAPD Markers in Riverine Water of Pakistan
Waqas Ahmad*, Muhammad Naeem
ABSTRACT
Identification and genetic diversity is important to explore biodiversity and conservation of species. Here we identified a commercially important fish Notopterus notopterus and analysed its genetic diversity. For the purpose of identification 15 specimens of N. notopterus were collected from Satluj, Ravi, Indus, Jehlum and Chenab rivers of Pakistan and barcoded with mitochondrial COI gene. BLAST analysis confirmed 100% identity of Notopterus notopterus with reference sequences belonging to NCBI GenBank database. Genetic diversity was analysed by using PCR amplification of 75 specimens of N. notopterus with five RAPD markers. RAPD markers data indicated high genetic diversity 0.5832 in Satluj while lowest genetic diversity 0.2173 in Chenab. High genetic polymorphism 60.52% observed in Satluj while lowest genetic polymorphism 23.07% observed in Chenab. Shannon’s Information Index (I) found 0.6281 in Satluj while 0.3162 in Chenab. Nei’s genetic diversity (h) among five riverine population was observed high in Satluj 0.5832 while lowest in Chenab 0.2173 which reflecting high genetic diversity in Satluj while lowest genetic diversity in Chenab. UPGMA dendrogram revealed that Indus and Jehlum riverine populations, and Satluj and Ravi populations of N. notopterus has close genetic similarity. Maximum likelihood tree revealed that N. notopterus identified from Pakistan has close genetic link with N. notopterus reported from Indo-Myanmar and India. Present study successfully reported molecular identification and genetic diversity of N. notopterus. Moreover, low genetic diversity reported in Chenab is matter of concern for fish resource managers and need to take constructive steps for its conservation.
Novelty Statement | DNA barcode base molecular identification and genetic diversity of N. notopterus evaluated from Satluj, Chenab, Jhelum, Ravi and Indus River of Pakistan through mitochondrial COI gene and RAPD markers reported first time from Pakistan. Low genetic diversity reported in Chenab River matter of concern for fish resource managers and need to take constructive steps for its conservation.
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December 2023
Vol.38, Iss. 2, Pages 137-236
