Immunomodulatory Effects of Calotropis procera on Human T-lymphocytes In Vitro
Immunomodulatory Effects of Calotropis procera on Human T-lymphocytes In Vitro
Sajida Batool, Iqra Ashraf, Shafaat Yar Khan, Sitara Shameem* and Breha Kazmi
ABSTRACT
Calotropis procera has been used in the traditional medicinal system for treating various diseases because of its phytochemical composition. In the present study, the methanolic leaf extract of C. procera was investigated for its immunomodulatory potential in vitro using T-lymphocytes. Phytohaemagglutinin (PHA) st https://researcherslinks.com/journals imulated T-lymphocytes were treated with extract of C. procera (1, 2, and 4 µg/mL) for 4, 8, 12, 24, and 48 h. Levels of pro-inflammatory cytokines (Interleukin-6 and Interleukin-8) were measured after 8, 12, and 24 h while levels of anti-inflammatory cytokines (Interleukin-10) were measured after 4 and 48-h via enzyme-linked immunosorbent assay (ELISA). C. procera treatment exhibited a significant decline in pro-inflammatory cytokines and an increase in anti-inflammatory cytokines on PHA stimulated T-cells in time-dependent and dose-dependent manner. These findings are suggesting the immunomodulatory potential of methanolic leaf extract obtained from C. procera that together with its other therapeutic properties can be used towards modulating the immune response.
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