Protective Effects of Oviductus Ranae Mediated HPA Axis Regulation on Depressive Model of Mice
Protective Effects of Oviductus Ranae Mediated HPA Axis Regulation on Depressive Model of Mice
Xiaowei Huang1, Chao Ma1, He Lin1, Yuchen Wang1, Yan Xu1, Guangfu Lv2* and Zhe Lin1*
ABSTRACT
Oviductus ranae (OR) is an animal-based traditional medicine. To explore its mechanism of antidepressant effect, healthy Institute of Cancer Research (ICR) male mice were divided into control, chronic unpredictable mild stress (CUMS), fluoxetine (3 mg/kg), OR800 (OR 800 mg/kg) and OR400 (OR 400 mg/kg) dose groups. After the last administration, the content of corticotropin-releasing hormone (CRH), adrenocorticotrophic hormone (ACTH) and corticosterone (CORT) in serum were detected. Hematoxylin and Eosin (H&E) staining was used to observe the hippocampal histomorphological changes. The protein levels of glucocorticoid receptor (GR), mineralocorticoid receptor (MR), brain-derived neurotrophic factor (BDNF), trosine kinase B (TrkB) and cyclic adenosine monophosphatec response element binding protein (CREB) in hippocampus were detected by western blotting. The results showed that, OR could significantly reduce the contents of CRH, ACTH and CORT in serum and improve the damage of hippocampal structure, and increase the expression levels of GR, MR, BDNF, TrkB and CREB in hippocampus. In conclusion, Oviductus ranae can reduce hypothalamic-pituitary-adrenal (HPA) axis hyperfunction induced by CUMS, increase the expression level of BDNF related signal pathway proteins, improve hippocampal tissue damage, and thus play an antidepressant role by regulating the negative feedback of HPA.
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