Evaluation of Pharmacognostic Features and Antimicrobial Activities of Dysphania botrys L.
Evaluation of Pharmacognostic Features and Antimicrobial Activities of Dysphania botrys L.
Muhammad Naeem Khan* and Asad Jan
ABSTRACT
Dysphania botrys L. is an annual herbaceous plant and has been utilized for the treatment of different ailments like asthma, cold, influenza, head ach, liver and digestive problems and healing of wounds. The aim of the study was to explore phytochemical constituents of methanolic crude extract (MCE) and various solvent fractions and in-vitro antimicrobial activities of whole plant of D. botrys. Qualitative phytochemical screening of MCE and solvent fractions of D. botrys showed the presence of alkaloids, phenols, flavonoids, saponins, tannins and sterols, however in n-hexane fraction (HxF) only flavonoids and saponins were detected. In quantitative analysis, among all the solvents, ethyl acetate fraction (EAF) had highest amount of phenol (27.4 mg/g), flavonoids (15.5 mg/g) and alkaloids (3.14 mg/g), while MCE displayed maximum amount of saponins (34.3 mg/g). In the proximate analysis, nitrogen freed extract was present in higher amount (38.45±0.83 %) followed by protein (30.26±0.72 %) while crude fibers were found least in amount (1.43±0.53 %). Among different minerals, reasonable amount of calcium (3268±0.53 μg/g), potassium (2873±0.71 μg/g), sodium (591±0.23 μg/g) and iron (223 ± 0.46 μg/g) were found while no cadmium and chromium was detected. MCE and EAF displayed considerable antibacterial activity against Xanthomonas campestris and Pseudomonas aeruginosa causing 12.6±0.54 mm and 20.6±0.53 mm zone of inhibition, respectively which was analogous to that of cefixime, used as standard drug. In case of antifungal activity MCE hindered the growth of Fusarium oxysporum effectively, causing 19.3±0.41 mm zone of inhibition, while the activity of other solvent was moderate.
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