Thecomplete blood count is powerful tool for the aquaculturist to monitor quality of water, related soil and to ensure the health status in response to feed, disease therapy and confirming the maturity. So the present study was designed to determine the hematological parameters of farm and river Chenab (wild) selected fish species including (Labeo rohita, Cirrhinus mrigala, Cyprinus carpio, Hypophthalmichthys molitrix, Ctenopharyngodon idella). Fish species selected for the current study is generally widely cultured in Pakistan and worldwide. Fish sampling was done by using different nets (river and farm) and blood sampling was immediately drawn from caudal peduncle by syringe and added in EDTA containing vial and shake well for proper mixing. Vials stored in ice containing boxes and carried to laboratory for total complete blood count. The water parameter was analyzed in laboratory by taking the sample of water and some (Temperature, DO) was measured in situ. Results of hematological analysis revealed that there is no significant (P > 0.05) difference in packed cell volume, mean cell volume and mean cell hemoglobin of different selected species adapted to similar environmental conditions. However, all other parameters like red blood cells, hemoglobin, mean cell hemoglobin concentration, white blood cells, lymphocytes, mid-range cells, granulocytes and other less important parameters including platelets, red cell distribution width-standard deviation, red cell distribution width-coefficient of variation, platelet distribution width, mean platelet volume, Plateletcrit and Platelet-Large cell ratio are differed significantly (P<0.05). Similar trend was observed in fishes of both selected sites “Farm” and “River. It was concluded that hematological variance can occur between animals of different species and same species of different habitat, but as the water parameter remains the same across the studies so current studies don’t show any significant difference.