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Fermentation Characteristics, In Vitro Nutrient Digestibility, and Methane Production of Oil Palm Frond-Based Complete Feed Silage Treated with Cellulase

Fermentation Characteristics, In Vitro Nutrient Digestibility, and Methane Production of Oil Palm Frond-Based Complete Feed Silage Treated with Cellulase

Budi Santoso*, Bambang Tjahyono Hariadi, Marlyn Nelce Lekitoo

Department of Animal Science, Faculty of Animal Science, University of Papua, Manokwari, West Papua 98314-Indonesia.

 
*Correspondence | Budi Santoso, Department of Animal Science, Faculty of Animal Science, University of Papua, Manokwari, West Papua 98314-Indonesia; Email: b.santoso@unipa.ac.id

ABSTRACT

This study examined the chemical composition, fermentation properties, nutritional digestibility, and CH4 generation of oil palm frond-based complete feed silage to assess the impact of a cellulase additive. In the complete randomized design experiment, six treatments (silage) were used: A, B, C, D, E, and F. Treatment A consisted of oil palm frond (40%), king grass (30%), cassava (10%), tofu waste (10%), molasses (7%), lactic acid bacteria (LAB) inoculant (3%), and cellulase 0 ml/kg. Treatment B comprised silage A + 3 ml cellulase/kg, while C used silage A + 6 ml cellulase/kg. Furthermore, treatment D consisted of palm frond (50%), king grass (20%), cassava (10%), tofu waste (10%), molasses (7%), LAB inoculant (3%), and cellulase 0 ml/kg. Treatment E used silage D + 3 ml cellulase/kg, while F was composed of silage D + 6 ml cellulase/kg. LAB inoculant added to the silage material was approximately 3.3 × 106 cfu/ml. The results showed that DM, NDF, ADF, and ADL contents in complete feed silage containing 40% oil palm frond with the addition of cellulase enzyme were descending (P<0.05) than without cellulase. Lactic acid concentrations were greater (P<0.01) in silages with 40% and 50% oil palm frond that were treated with 4 ml/kg and 6 ml/kg cellulase than in silages that did not receive cellulase treatment. Additionally, silage treated with cellulase (B and C) had higher IVDMD, IVOMD, and IVNDF (P<0.01) than silage treated without cellulase treatment (A). Based on the findings, it was determined that cellulase enzymes efficiently decreased the crude fiber fraction in complete feed silage generated from palm frond, while also enhancing the quality of fermentation and nutrient digestibility.
 
Keywords | Fermentation, Methane, Oil palm frond, Ruminant, Silage

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Advances in Animal and Veterinary Sciences

July

Vol. 12, Iss. 7, pp. 1206-1409

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