ELISA Based Monitoring of Citrus Tristeza Virus in Declining Orchards of Sargodha, Pakistan
ELISA Based Monitoring of Citrus Tristeza Virus in Declining Orchards of Sargodha, Pakistan
Talha Shafique1, Mustansar Mubeen2, Yasir Iftikhar2, Qaiser Shakeel3*, Muhammad Ahmad Zeshan2, Haseeb Ahmed Lalika4, Malik Abd-ur-Rehman5, Atta ur Rehman2, Muhammad Irfan Zafar6 and Farwa Seemab2
ABSTRACT
This study assesses the current status of citrus tristeza virus (CTV) in three tehsils of the Sargodha district: Kot Momin, Sargodha, and Bhalwal. The incidence and severity of CTV were evaluated using three citrus varieties: Musambi, Feutrel’s Early, and Kinnow. Disease incidence was calculated using the ELISA test, while disease severity was determined by counting the number of pits per square inch. The results revealed significant variation in CTV incidence and severity across the three citrus varieties. Musambi exhibited the highest incidence rate at 4.5, followed by Feutrel’s Early at 2.89 and Kinnow at 1.71. Musambi also had the highest severity score at 53.5 pits per square inch, followed by Feutrel’s Early at 27.5 and Kinnow at 11.5. Geographical analysis of CTV incidence indicated that Kot Momin had the highest incidence rate at 4.73, followed by Bhalwal at 2.89 and Sargodha at 1.9. Correspondingly, Kot Momin also showed the highest severity score at 33.5 pits per square inch, with Bhalwal and Sargodha recording 25.5 and 12.5, respectively. The research provides deep insights and understanding for the identification of high-risk areas in Punjab and susceptible varieties, which are crucial for developing effective CTV mitigation strategies. This research contributes to the existing body of knowledge by presenting novel insights into the geographical and varietal differences in CTV impact, paving the way for future studies and interventions.
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