Antibiotic Resistance Coding Genes in Klebsiella pneumoniae from Clinical Cats in Bogor Indonesia
Antibiotic Resistance Coding Genes in Klebsiella pneumoniae from Clinical Cats in Bogor Indonesia
Safika1*, Ni Luh Putu Ika Mayasari1, Juliadi Ramadhan2
ABSTRACT
Infectious agents like as viruses, bacteria, fungi, and parasites can cause feline respiratory conditions. Antibiotics can be used to treat diseases caused on by bacterial organisms. This research aims to determine antibiotic resistance and the gene coding for antibiotic resistance in Klebsiella pneumoniae isolated from clinical cats in Bogor, Indonesia. This study’s total sample comprised of 58 clinical cat laryngeal swabs. Samples were isolated and biochemically and molecularly identified. The Kirby-Bauer disk diffusion method was used to test positive isolates, followed by PCR method to discover the resistance coding genes. The results showed that ampicillin showed 76.0% resistance to Klebsiella pneumoniae, followed by oxytetracycline (72.0%) and tetracycline (68.0%), enrofloxacin (52.0%), and gentamicin (44.0%). All Klebsiella pneumoniae isolates carried the blaTEM gene, 57.20 % contained the tetA gene, 30.40 % contained the blaSHV gene, 33.3 % contained the aac3-IV gene, and 28.50 % contained the qnrS gene. In this investigation, all Klebsiella pneumoniae were isolates were resistant to antibiotics and were categorized as multi drug resistant (MDR), demonstrating variable proportions of antibiotic resistance coding genes. Antibiotic resistance in Klebsiella pneumoniae has been detected in the majority of veterinary clinics in Bogor, although at varying degrees in each group. Resistance gene activity against each antibiotic can measure both the phenotype and genotype of Klebsiella pneumoniae resistance development.
Keywords | Antibiotic resistance, Cats, Feline respiratory, Klebsiella pneumoniae, Multi drug resistant
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March 2023
Vol. 11, Iss. 3, Pages 355-516
