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Effect of Green Tea Extract (Camellia sinensis) on Fertility Indicators of Post-Thawed Bull Spermatozoa

Effect of Green Tea Extract (Camellia sinensis) on Fertility Indicators of Post-Thawed Bull Spermatozoa

Hamayun Khan1,*, Momen Khan2, Muhammad Subhan Qureshi3, Shakoor Ahmad1, Ali Gohar4, Hameed Ullah5, FarmanUllah6, Arab Hussain7, Pershotam Khatri8, Said Sajjad Ali Shah1, Hamid Rehman1 and Azmatullah Khan1

1Department of Animal Health, The University of Agriculture, Peshawar, Pakistan
2 Livestock and Dairy Development, Khyber Pakhtunkhwa, Pakistan
3 Department of Livestock Management ,The University of Agriculture, Peshawar, Pakistan
4 College of Veterinary Sciences and Animal Husbandry, Abdul Wali Khan University,
Garden Campus, Mardan, Pakistan
5Department of Veterinary Medicine, Sind Agriculture University, Tando Jam, Pakistan
6Department of Animal Breeding and Genetics , Faculty of Veterinary and Animal Sciences Lasbella University of Agriculture, Water and Marine Sciences, Uthal, Pakistan
7Department of Animal Sciences, Govt. Postgraduate College, Charsadda, Pakistan
8Departement of Animal Reproduction, Sind Agriculture University, Tando Jam, Pakistan

*      Corresponding author: 


Green tea extract is plant–dervied natural antioxidant. The objective of this study was to explore the antioxidative effect of green tea extract on the cryopreserved bull semen. Experiments were carried out on Achai breeding bulls of similar age at the Semen Production Unit, Harichand, District Charsadda, Khyber Pakhtunkhwa. Collection of semen was done using artificial vagina (42°C) for three weeks. Semen was extended in egg yolk extender at various inclusion levels (0.0%, 0.25%, 0.5%, 0.75 and 1.0%) of green tea extract. Testing was consummated for quality characteristics of post-thawed spermatozoa such as motility, viability and plasma membrane integrity. The data was statistically analyzed with one-way analysis of variance using SPSS. The highest significant (P<0.05) response was obtained in extenders containing 0.75% green tea extract for all studied parameters. Based on the findings of the current study, it can be concluded that supplementation of green tea extract in semen extender showed significant response on the post-thawed spermatozoa motility, viability and membrane integrity of Achai bull.

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Pakistan Journal of Zoology


Pakistan J. Zool., Vol. 56, Iss. 4, pp. 1501-2000


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