Virological and Serological Investigations on Avian Pneumovirus in Turkeys and Chickens
Virological and Serological Investigations on Avian Pneumovirus in Turkeys and Chickens
Madboulyl , H. M.; Tamami , S.M.; Abd Elmoneml , A.S.; Husseinl , A.S. and Arafa2, A.M.
ABSTRACT
Avian Pneumovirus (APV), which is termed turkey rhinotracheitis (TRT), is successfully isolated from 5 out of 8 samples of nasal discharge and 10% homogenates of nasal sinuses collected from young turkey poults of unvaccinated flocks by passage on SPF-ECE of 5-7 days old embryos via yolk sac route. Characterization of isolated virus was done by using RT-PCR where the 5 isolates were positive for APV and produced a specific 315 bp products. Serological survey for APV antibodies in serum and egg yolk samples collected from non-vaccinated flocks (608 serum samples were obtained from broiler chickens from El-Fayoum, Beni suef and Giza, 40 turkey poults serum samples from Al Behira and El Monofia, 278 chicken egg yolk samples from El-Monofia, Dommiat, Alexandria and Al Behira and 675 turkey egg yolk samples from El-Fayoum and Beni suef Governorates) were tested by using ELISA. The percentages of positive reactors of chicken sera and egg yolk samples were 57.4% and 74.4%, while it were 77.5% and 83.2% respectively in turkey sera and egg yolk. The APV disease was recorded in young chicks and turkey poults showed typical symptoms and APV was isolated regardless the presence of high level of maternal derived antibody in their sera. This denotes the protection against APV independent on the presence of high levels of maternal antibodies.
To share on other social networks, click on any share button. What are these?
