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In vivo Anti-Inflammatory and Anti-Arthritic Potential of Ethanolic Acacia modesta Extract on CFA-Induced Adjuvant Arthritic Rats

In vivo Anti-Inflammatory and Anti-Arthritic Potential of Ethanolic Acacia modesta Extract on CFA-Induced Adjuvant Arthritic Rats

Farah Abid1,2, Mohammad Saleem1,3*, Tahir Maqbool4, Tania Ahmed Shakoori5, Faheem Hadi4, Tahir Muhammad4, Saira Aftab4, Yasir Hassan7 and Shabana Akhtar4,6*

1Faculty of Pharmacy, Government College University, Faisalabad, Pakistan
2Faculty of Pharmacy, The University of Lahore, Lahore, Pakistan
3Faculty of Pharmacy, University of the Punjab, Lahore, Pakistan
4Centre of Research in Molecular Medicine, Institute of Molecular Biology and Biotechnology, The University of Lahore, Lahore, Pakistan
5Department of Physiology and Cell Biology, University of Health Sciences, Lahore
6School of Medical Sciences, University of Bradford, UK
7Lahore Business School, The University of Lahore, Lahore, Pakistan
 
* Corresponding author: s.akhtar133@bradford.ac.uk, saleem2978@hotmail.com

ABSTRACT

The objective of the present study was to investigate the role of Acacia modesta extract whole plant extract (E-AM) in process of inflammation and also in arthritis by using animal model of rat. Thirty-six healthy wister rats were chosen for in vivo experiments and divided into six groups. Arthritis was induced in rats using Complete Freund’s Adjuvant (CFA) and treated with different concentrations of E-AM (250, 500 or 750 mg/kg), and with standard treatment, piroxicam (10 mg/kg). Antioxidant assays (superoxide dismutase and catalase) and histopathological examination were performed to determine the values of various parameters of inflammation and arthritis. Real-Time PCR technique was applied to analyze the expression levels of different cytokines and interleukins involved in inflammation. Real-Time PCR results showed a down-regulation of TNF-α, COX-2, IL-6, IL-1β, NF-κβ1and up-regulation of IL-4, IL-10 and I-κB genes. Levels of antioxidants, superoxide dismutase and catalase were determined to be significantly higher (p<0.05) in the group treated with E-AM compared to the arthritic group. In addition, histopathological examination indicated reduction in the index of C-reactive protein, rheumatoid factor and bone and cartilage erosion after treatment with E-AM. Collectively, our findings suggest that E-AM extract might have potential to perform anti-inflammatory and anti-arthritic activity which could have beneficial impact for the patients associated with arthritis.

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Pakistan Journal of Zoology

February

Vol. 55, Iss. 1, Pages 1-500

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