Storage of Bacteriophages at 4°C Leads to no Loss in Their Titer after One Year
Storage of Bacteriophages at 4°C Leads to no Loss in Their Titer after One Year
Iqbal Ahmad Alvi, Muhammad Asif, Rabia Tabassum, Zaigham Abbas and Shafiq ur Rehman*
ABSTRACT
The storage of bacteriophages is tricky as no ideal common practice is available and the storage conditions may differ from phage to phage. There is disparity among the scientific community regarding bacteriophage storage conditions and various conditions (4 oC, -20 oC, -80 oC and liquid nitrogen freezing at -196°C) are used by different laboratories. In this study, the storage stability of five bacteriophages, JHP, RLP, RSP, SaPL, and IttPL, at distinct temperatures for complete one year is described. Our results indicated that storage of bacteriophages at 4oC was most appropriate, followed by storage at -20oC and -80oC as no drop in viral titer was witnesses in bacteriophage samples stored at 4oC for up to one year. The results of this research indicated, that a standard refrigeration is suitable and economical means of long term storage with no fall of phage activity.
To share on other social networks, click on any share button. What are these?
December 2018
Vol. 50, Iss. 6, Pages 1999-2398
