Clinical Isolation and Genetic Established and Saprophytic Bacteria During Normal Estrus Cycle of Cow
Clinical Isolation and Genetic Established and Saprophytic Bacteria During Normal Estrus Cycle of Cow
Hayder A.H. Al-Mutar*, Baqer J. Hasan, Khawla Abbas Hussein, Souhayla Oneeis Hussain
ABSTRACT
There are many cases of vaginitis and metritis occurring in animals farms specially in cows In majority of these cases, the infection remained unnoticed caused by proliferation of saprophytic bacteria that present normally in mucosal membrane of vaginal. These infections predispose cow more susceptible to infection and bacterial proliferation during the stages of estrus periods (proestrus, estrus, diestrus and metestrus). Here, we investigated the presence of saprophytic bacteria in vaginal smears, and characterize them using PCR. All vaginal swabs collected from 43 sexually matured cows were subjected to Stuart transport media, before planted in blood agar media to identify the number of colonies counted. The bacteria was confirmed by Gram’s staining and biochemical tests. The PCR products from the bacterial samples were genetically analyzed (Al-Mutar et al., 2018). The result show that the most bacterial present in bovine vagina were Streptococcus thermophilus and Streptococcus uberis (92.65%) while the lowest percent of bacteria present in bovine vagina was Corynebacterium frankenforstense (78.8%). The other saprophytic bacteria were Streptococcus lutetiensis, Klebsiells pneumoniae, Escherichia coli and Staphylococcus aurreus were present in a percentage from 94.61%, 93.25%, 80.5% and 78.69%. respectively. The study concluded that there are many types of Saprophytic bacterial proliferation during estrus cycle inside vagina and uterus of cows with a variation of different countries, bread and age.
Keywords | Saprophytic bacteria, Estrus cycle, Cows, PCR, Clinical isolation
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November 2024
Vol. 12, Sp. Iss. 1
