Effect of Hedyotis diffusa Polysaccharide on Apoptosis of Thyroid Cancer Cells
Effect of Hedyotis diffusa Polysaccharide on Apoptosis of Thyroid Cancer Cells
Lin Lu
ABSTRACT
The objective of this study was to investigate the effect and mechanism of Hedyotis diffusa polysaccharide (HDP) on apoptosis of thyroid cancer cell line. TPC-1 cells were treated with HDP (final concentration 1 mg/mL, 2 mg/mL and 4 mg/mL), PI3K/AKT signal pathway inhibitor LY294002 (20 μmol/L) and LY294002 + HDP (4 mg/ml HDP and 20 μmol/L LY294002). Cell viability and apoptosis rate were detected by MTT and flow cytometry, respectively. The expression of Cyt-C, Bcl-2, Bax, cleaved Caspase-3, PI3K, AKT and p-AKT were detected by Western blotting. We found that activity of TPC-1 cells was inhibited by different concentrations of Polysaccharide from H. diffusa, which was time and concentration dependent (P<0.05). When TPC-1 cells were treated with polysaccharide of H. diffusa for 48 h, the apoptosis rate increased, the expression of Bcl-2, PI3K and p-AKT decreased, the expression of Cyt-C, Bax and cleaved caspase 3 increased significantly (P<0.05). LY294002 could significantly inhibit the activity of TPC-1 cells, promote apoptosis, down regulate the expression of Bcl-2, PI3K and p-AKT, up regulate the expression of Cyt-C, Bax and cleaved caspase 3, and significantly enhance the effect of Polysaccharide from H. diffusa on the activity, apoptosis of TPC-1 cells and the expression of Cyt-C, Bcl-2, Bax, cleaved caspase 3, PI3K, AKT and p-AKT (P < 0.05). We concluded HDP can promote the apoptosis of thyroid cancer cells by inhibiting the PI3K/AKT signal pathway.
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